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FEBS Lett. 1993 Jul 19;327(1):71-4.

Phosphorylation of elongation factor-2 from the lepidopteran insect, Spodoptera frugiperda.

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  • 1Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.


In mammalian cells, protein synthesis can be regulated at the level of elongation by the phosphorylation of elongation factor 2 (eEF-2) by a highly specific Ca2+/calmodulin-dependent kinase. In this report, we show that eEF-2 from a cell line derived from the insect, Spodoptera frugiperda, is a substrate for mammalian eEF-2 kinase and that phosphorylation is Ca(2+)-dependent. Furthermore, two-dimensional peptide mapping shows that the kinase phosphorylates the same sites in Spodoptera eEF-2 as those phosphorylated in the rabbit protein. However, we were unable to detect an eEF-2 kinase in Spodoptera cells.

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