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Cell. 1993 Jul 16;74(1):205-14.

Mammalian Ras interacts directly with the serine/threonine kinase Raf.

Author information

1
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

Abstract

We have identified proteins that interact with H-Ras using a two hybrid system screen of a mouse cDNA library. Approximately 50% of the clones identified encoded portions of the c-Raf and A-Raf serine/threonine kinases. Overlaps among these clones define a conserved 81 residue region of the N-terminus of Raf as the Ras interaction region. We show that Raf interacts with wild-type and activated Ras, but not with an effector domain mutant of Ras or with a dominant-interfering Ras mutant. Using purified bacterially expressed fusion proteins, we show, furthermore, that Ras and the N-terminal region of Raf associate directly in vitro and that this interaction is dependent on GTP bound to Ras.

PMID:
8334704
[Indexed for MEDLINE]

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