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Biotechniques. 1993 Jun;14(6):920-4.

Elimination of false positives that arise in using the two-hybrid system.

Author information

1
Department of Microbiology, State University of New York, Stony Brook 11794.

Abstract

The two-hybrid system is a genetic method to identify proteins that interact with a specific target protein, which is expressed in yeast as a hybrid with a DNA-binding domain. Use of this method entails transforming yeast, both with this DNA-binding domain hybrid and with a library of activation domain hybrids, followed by screening for transcriptional activation of a reporter gene. In addition to proteins that interact with the target protein, a number of false positives are identified. We provide possible explanations for these false positives along with rapid assays to eliminate them.

PMID:
8333960
[Indexed for MEDLINE]

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