Send to

Choose Destination
Somat Cell Mol Genet. 1993 May;19(3):265-74.

Neurofibromatosis type 1 gene product (neurofibromin) associates with microtubules.

Author information

Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109.


The neurofibromatosis type 1 (NF1) gene was recently identified by positional cloning and found to encode a protein with structural and functional homology to mammalian and yeast GTPase-activating proteins (GAPs). Using antibodies directed against the NF1 gene product, a protein of approximately 250 kDa was identified and termed neurofibromin. Double-indirect immunofluorescent labeling with anti-neurofibromin and anti-tubulin antibodies demonstrates that neurofibromin associates with cytoplasmic microtubules. Immunoblotting of microtubule-enriched cytoplasmic fractions, using antibodies generated against neurofibromin, shows that neurofibromin copurifies with microtubules. When portions of neurofibromin are expressed in Sf9 insect cells they associate with polymerized microtubules; furthermore, the critical residues for this interaction reside within the GAP-related domain of neurofibromin. The unexpected association of neurofibromin with microtubules suggests that neurofibromin is involved in microtubule-mediated intracellular signal transduction pathways.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for MLibrary (Deep Blue)
Loading ...
Support Center