[Properties of NAD-pyrophosphorylase of the nuclei of liver cells of chickens]

H I Fomenko; S I Shuhevych; A H Khalmuradov

[Properties of NAD-pyrophosphorylase of the nuclei of liver cells of chickens]

Ukr Biokhim Zh. 1975 Jan-Feb;47(1):8-11.

[Article in Ukrainian]

Authors

H I Fomenko, S I Shuhevych, A H Khalmuradov

PMID: 833

Abstract

Certain characteristics of chicken liver cells nuclei NAD-pyrophosphorylase (NMN-adenylytranspherase, EC 2.7.7.1) were investigated. It was established that NAD-pyrophosphorylase activity optimum pH is within interval of 7.0-7.5; temperature optimum - 38-39 degrees C; factor Q10 is equal to 2. Enzyme activation energy, inactivation energy and enthalpy were calculated; apparent Km values of NAD-pyrophosphorylase with respect to NMN and ATP are equal to 1.62-10(-7) M and 2.61-10(-7) M, respectively.

Publication types

English Abstract

MeSH terms

Adenosine Triphosphate
Animals
Cell Nucleus / enzymology
Chickens
Hydrogen-Ion Concentration
Liver / enzymology*
Nicotinamide Mononucleotide
Nucleotidyltransferases / metabolism*
Temperature

Substances

Nicotinamide Mononucleotide
Adenosine Triphosphate
Nucleotidyltransferases