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FEBS Lett. 1994 Feb 7;338(3):290-4.

Cloning of eggplant hypocotyl cDNAs encoding cytochromes P450 belonging to a novel family (CYP77).

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Central Laboratory for Key Technology, Kirin Brewery Co. Ltd, Yokohama, Japan.


From eggplant hypocotyl tissues we have cloned two closely related cDNAs encoding cytochromes P450 (P450s) by PCR amplification using a primer designed based on the highly conserved sequence among the known eggplant P450s. One cDNA lacks the NH2-terminal short sequence that is present in the other, full-length cDNA. The two predicted protein sequences are 71% identical with each other and show less than 30% identity with any other known P450s. It is concluded that these P450s, which are termed CYP77A1 and -A2, belong to a hitherto unknown P450 family.

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