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FEBS Lett. 1994 Feb 7;338(3):290-4.

Cloning of eggplant hypocotyl cDNAs encoding cytochromes P450 belonging to a novel family (CYP77).

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1
Central Laboratory for Key Technology, Kirin Brewery Co. Ltd, Yokohama, Japan.

Abstract

From eggplant hypocotyl tissues we have cloned two closely related cDNAs encoding cytochromes P450 (P450s) by PCR amplification using a primer designed based on the highly conserved sequence among the known eggplant P450s. One cDNA lacks the NH2-terminal short sequence that is present in the other, full-length cDNA. The two predicted protein sequences are 71% identical with each other and show less than 30% identity with any other known P450s. It is concluded that these P450s, which are termed CYP77A1 and -A2, belong to a hitherto unknown P450 family.

PMID:
8307197
DOI:
10.1016/0014-5793(94)80286-6
[Indexed for MEDLINE]
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