Abstract
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / growth & development
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Cell Division
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Cell Separation
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Escherichia coli / genetics*
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Fluorescence
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Gene Expression*
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Green Fluorescent Proteins
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Luminescent Proteins / analysis*
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Luminescent Proteins / genetics
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Microscopy, Fluorescence
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Molecular Sequence Data
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Neurons / chemistry*
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Oligodeoxyribonucleotides
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Recombinant Proteins
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Spectrometry, Fluorescence
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Transformation, Genetic
Substances
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Luminescent Proteins
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Oligodeoxyribonucleotides
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Recombinant Proteins
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Green Fluorescent Proteins