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Gene. 1993 Dec 22;136(1-2):355-60.

A human gene that shows identity with the gene encoding the angiotensin receptor is located on chromosome 11.

Author information

1
Addiction Research Foundation, Toronto, Ontario, Canada.

Abstract

We report the cloning of a gene, intronless in its coding region, which we have named APJ. This gene was cloned using the polymerase chain reaction (PCR), with a set of primers designed on the basis of the conservation that members of G protein-coupled receptors (GPCR) have in their transmembrane (TM) regions. The putative receptor protein, APJ, shares closest identity to the angiotensin receptor (AT1) ranging from 40 to 50% in the hydrophobic TM regions of these receptors. The transcripts for this gene were detected in many regions of the brain. PCR analysis of somatic cell lines found APJ-related sequences to be only present on chromosome 11, and high-resolution mapping by fluorescence in situ hybridization (FISH) sublocalized APJ on band q12.

PMID:
8294032
DOI:
10.1016/0378-1119(93)90495-o
[Indexed for MEDLINE]

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