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Gene. 1993 Dec 22;136(1-2):253-5.

A counterselectable pACYC184-based lacZ alpha-complementing plasmid vector with novel multiple cloning sites; construction of chromosomal deletions in Klebsiella pneumoniae.

Author information

1
AFRC Institute of Plant Science Research, Nitrogen Fixation Laboratory, University of Sussex, Brighton, UK.

Abstract

We have constructed a series of small, chloramphenicol-resistance-encoding, lacZ alpha-complementing vectors with novel multiple cloning sites, based on the pACYC184 replicon. The sacB gene of Bacillus subtilis, which is lethal to Gram- organisms in the presence of sucrose, was cloned into one of these, giving the counterselectable vector pSG335. This was used to substitute a streptomycin-resistance-encoding cassette for the ntrBC genes in the Klebsiella pneumoniae chromosome.

PMID:
8294011
DOI:
10.1016/0378-1119(93)90474-h
[Indexed for MEDLINE]

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