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J Histochem Cytochem. 1994 Feb;42(2):155-66.

Ultrastructural localization of gangliosides; GM1 is concentrated in caveolae.

Author information

1
European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

The ultrastructural distribution of the ganglioside GM1 was investigated in A431 cells. After fixation, the cells were frozen in liquid nitrogen, freeze-substituted, and then embedded in Lowicryl resin at -45 degrees C. By use of the cholera toxin-binding subunit adsorbed to gold as a specific probe to label on the sections, GM1 was shown to be present in endocytic organelles, in the trans-Golgi network, and on the plasma membrane, but was not detectable in the endoplasmic reticulum. GM1 was not distributed uniformly over the plasma membrane but was concentrated approximately four-fold in non-coated invaginations. These were identified as caveolae by labeling frozen sections of cholera toxin-gold surface-labeled cells with antibodies to VIP-21/caveolin. The results strengthen the functional analogy between caveolae and sorting domains of the TGN in polarized epithelial cells.

PMID:
8288861
DOI:
10.1177/42.2.8288861
[Indexed for MEDLINE]

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