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Eur J Surg. 1993 Oct;159(10):535-9.

Effect of sepsis-related cytokines on lipid synthesis by isolated human hepatocytes.

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1
Department of Surgery, U. Complutense de Madrid, Ciudad Universitaria, Spain.

Abstract

OBJECTIVE:

To investigate the effect of tumour necrosis factor-alpha (TNF-alpha) and other sepsis related cytokines on the synthesis of several lipid fractions by human hepatocytes.

DESIGN:

Laboratory study.

SETTING:

University departments of surgery and biochemistry.

MATERIAL:

Livers from eight donors of other organs.

MAIN OUTCOME MEASURES:

Effect of TNF-alpha, interleukin-1 (IL-1), and interleukin-6 (IL-6) on the synthesis of triacylglycerol and phospholipids by freshly isolated human hepatocytes.

RESULTS:

All three cytokines, particularly TNF-alpha, inhibited the incorporation of D-glucose uniformly labelled with 14C (D-(U-14C) glucose) into phosphatidylcholine. The incorporation of D-(U-14C) glucose into triacylglycerol and free fatty acids was significantly stimulated by TNF-alpha and IL-1, but not by IL-6. When palmitate uniformly labelled with 14C was used as the radiolabelled precursor, TNF-alpha stimulated the synthesis of both triacylglycerol and phosphatidylcholine, neither of which was affected by IL-1 or IL-6.

CONCLUSION:

Changes that are induced by cytokines in lipid synthesis by hepatocytes can contribute to the impairment of lipid metabolism seen in septic patients. TNF-alpha seemed to have more effect than the other two cytokines.

PMID:
8286511
[Indexed for MEDLINE]
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