Send to

Choose Destination
J Med Virol. 1993 Oct;41(2):159-64.

Novel assay for the detection of immunoglobulin G antihuman immunodeficiency virus in untreated saliva and urine.

Author information

Virus Reference Division, Central Public Health Laboratory, London, United Kingdom.


Epidemiological evidence and laboratory studies indicate that human immunodeficiency virus type 1 (HIV 1) is rarely, if ever, transmitted in saliva or urine. In that both specimens are easy to collect, each may be a useful alternative to serum specimens for anti-HIV screening. A rapid, simple, and robust IgG-capture enzyme-linked immunosorbent assay (GACELISA) suitable for the detection of anti-HIV 1 and 2 in saliva and urine was developed. Following optimisation of the assay, 177 salivary and 568 urine specimens collected from individuals of known serostatus were investigated. The assay was 100% sensitive on 50 salivary (median OD/CO = 8.9) and 126 urinary (median OD/CO = 8.6) specimens collected from anti-HIV-positive patients. The specificity was 100% on 127 salivary specimens (median OD/CO = 0.37) and 422 urinary specimens (median OD/CO = 0.39) collected from anti-HIV-negative individuals. These findings demonstrate that GACELISA HIV 1 + 2 tests on saliva or on urine are an accurate alternative to a conventional anti-HIV test of blood. This assay is satisfactory for surveillance purposes and, with appropriate precautions, could be used clinically.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center