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Nucleic Acids Res. 1993 Nov 11;21(22):5192-7.

In vivo cloning of PCR products in E. coli.

Author information

1
Johns Hopkins Oncology Center, Baltimore, MD 21231.

Abstract

This report describes an efficient method to clone PCR products exploiting endogenous Escherichia coli enzymatic activities. PCR products are engineered to contain terminal sequences identical to sequences at the two ends of a linearized vector. PCR products and vector DNA are then simply co-transfected into E. coli strain JC8679, obviating the requirement for enzymatic treatment of the PCR product or in vitro ligation. The high rate of homologous recombination in this strain results in efficient incorporation of the insert into the vector, a process we refer to as in vivo cloning (IVC).

PMID:
8255776
PMCID:
PMC310636
DOI:
10.1093/nar/21.22.5192
[Indexed for MEDLINE]
Free PMC Article

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