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Nature. 1993 Dec 9;366(6455):537-43.

Crystal structure of a bacterial non-haem iron hydroxylase that catalyses the biological oxidation of methane.

Author information

1
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.

Abstract

The 2.2 A crystal structure of the 251K alpha 2 beta 2 gamma 2 dimeric hydroxylase protein of methane monooxygenase from Methylococcus capsulatus (Bath) reveals the geometry of the catalytic di-iron core. The two iron atoms are bridged by exogenous hydroxide and acetate ligands and further coordinated by four glutamate residues, two histidine residues and a water molecule. The dinuclear iron centre lies in a hydrophobic active-site cavity for binding methane. An extended canyon runs between alpha beta pairs, which have many long alpha-helices, for possible docking of the reductase and coupling proteins required for catalysis.

PMID:
8255292
DOI:
10.1038/366537a0
[Indexed for MEDLINE]

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