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Arch Dermatol. 1993 Dec;129(12):1589-93.

Rapid diagnosis of coccidioidomycosis with a DNA probe to ribosomal RNA.

Author information

1
Dermatology Service, Walter Reed Army Medical Center, Washington, DC.

Abstract

BACKGROUND:

Although nucleic acid probe hybridization assays were previously exclusively used as a tool in the research setting, such assays have recently become commercially available for the detection of a variety of infectious microorganisms.

OBSERVATIONS:

We used a commercially available DNA hybridization probe test that targets organism-specific ribosomal RNA sequences to rapidly diagnose a patient with disseminated coccidioidomycosis. The natural amplification inherent to such DNA:RNA probe systems obviates the need for electrophoretic separation and amplification steps, which are often required in more traditional DNA:DNA probe assays. With this probe, culture confirmation was obtained within 48 hours after the clinical specimens were received.

CONCLUSION:

Rapid DNA hybridization probe techniques have wide application in infectious diseases, especially those characterized by slow culture growth of pathogens such as deep fungi and atypical mycobacteria.

PMID:
8250580
[Indexed for MEDLINE]

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