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Anal Biochem. 1993 Oct;214(1):138-41.

Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green.

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1
Department of Medical and Physiological Chemistry, Biomedical Center, Uppsala, Sweden.

Abstract

An assay for protein-bound phosphate with a capacity to determine 100 pmol of phosphate is described. It is based on the combination of two well known methods: the alkaline hydrolysis of phosphate from seryl and threonyl residues in phosphoproteins and the quantification of the released phosphate by the use of malachite green and phosphomolybdate.

PMID:
8250216
DOI:
10.1006/abio.1993.1468
[Indexed for MEDLINE]

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