Format

Send to

Choose Destination
Biochemistry. 1993 Dec 7;32(48):13040-5.

Evidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanism.

Author information

1
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.

Abstract

The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca2+, Mn2+, Co2+, and Cd2+ correlate well with the pKa's of these ions in water. This relationship between the pKa's and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.

PMID:
8241158
DOI:
10.1021/bi00211a013
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center