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Plasmid. 1993 Sep;30(2):170-5.

Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation.

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1
Department of Microbiology, University of Georgia, Athens 30602.

Abstract

Mycoplasma pneumoniae was transformed with the Staphylococcus aureus transposon Tn4001 by electroporation. A transformation frequency of 10(-3) to 10(-5)/colony-forming unit was observed using 30.0 microgram plasmid DNA and 10(7)-10(8) M. pneumoniae colony-forming units. DNA hybridization analyses using standard and pulsed field agarose gel electrophoresis confirmed chromosomal insertion of the transposon, apparently by a transpositional mechanism into random sites. These studies demonstrate the functionality of Tn4001 in M. pneumoniae and suggest its potential as a genetic tool in this mycoplasma.

PMID:
8234492
DOI:
10.1006/plas.1993.1047
[Indexed for MEDLINE]
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