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J Am Acad Dermatol. 1993 Nov;29(5 Pt 1):715-22.

Merkel cell carcinoma: in vitro and in vivo characteristics of a new cell line.

Author information

1
Specialized Cancer Center, University of Illinois, Chicago 60612.

Abstract

BACKGROUND:

Few studies exist that describe Merkel cell carcinoma (MCC) growth characteristics in vitro, in vivo, or both.

OBJECTIVE:

Our purpose was to evaluate the pathologic features of MCC implanted into athymic mice and to determine cytogenetic abnormalities in the established cell line.

METHODS:

Tumor tissues from a patient with MCC were grown in culture. Cultured cells were karyotyped and inoculated subcutaneously into athymic mice. Nude mouse tumors were re-implanted into other athymic mice. Tissues from the primary skin tumor and the nude mouse tumor were processed for light and electron microscopy and immunocytochemistry.

RESULTS:

The cell line showed a doubling time of 64.8 hours. Xenografts of 4 x 10(6) cells produced tumors in athymic mice with a doubling time of 16.1 days. The nude mouse tumors showed pathologic features similar to those of the primary skin tumor. Cytogenetic studies showed a t(1;17) (p36;q21) translocation in 100% of the cells.

CONCLUSION:

MCC implanted into athymic mice retained the pathologic features of the primary skin tumor and behaved aggressively. The t(1;17) (p36;q21) translocation may be a marker of an aggressive phenotype.

PMID:
8227544
DOI:
10.1016/0190-9622(93)70236-m
[Indexed for MEDLINE]

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