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Free Radic Biol Med. 1993 Oct;15(4):447-51.

Luminol and lucigenin as detectors for O2.-.

Author information

1
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.

Abstract

Univalent oxidation of luminol and univalent reduction of lucigenin must precede reaction with O2.- if that reaction is to lead to luminescence. The assumption that luminol or lucigenin, per se, reacts with O2.- in a way leading to luminescence is incorrect, and leads to misinterpretation of results. The chemical reactions leading to the O2(.-)-dependent luminescences of luminol and of lucigenin are discussed.

PMID:
8225026
DOI:
10.1016/0891-5849(93)90044-u
[Indexed for MEDLINE]

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