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Experientia. 1993 Oct 15;49(10):893-901.

Kinetic analysis of lactate dehydrogenase using integrated rate equations.

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Department of Chemistry and Biochemistry, Utah State University, Logan 84322.


The reaction catalyzed by lactate dehydrogenase was analyzed under fully second-order conditions using integrated rate equations. A two-step regression analysis was utilized to fit twenty-one progress curves repeated in sextuplicate to the general mechanism second-order integrated rate equation with additional terms of substrate inhibition. The fitting error was less than one percent. The resulting kinetic constants support a ternary complex mechanism; in no case were constants supporting another mechanism predicted. The inhibition constant for oxamate was also determined.

[Indexed for MEDLINE]

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