A new infectious mammary tumor virus in the milk of mice implanted with C4 hyperplastic alveolar nodules

Eur J Immunol. 1993 Nov;23(11):2765-9. doi: 10.1002/eji.1830231107.

Abstract

We have previously characterized an infectious mouse mammary tumor virus [(MMTV(SW)] which induces a strong superantigen response in vivo. Here we describe the isolation and characterization of MMTV(C4) which was derived from milk of mice implanted with hyperplastic alveolar nodules. MMTV(C4) stimulates V beta 2 expressing T cells after local injection in vivo. Comparison with known open reading frame (orf) sequences revealed high homology to Mtv-6, an endogenous virus interacting with V beta 3-expressing T cells. The carboxyl-terminal amino acids were, however, altered. High homology including the carboxyl-terminal orf amino acids were found with MMTV(C3H-K). We show here that MMTV(C3H-K) has lost its superantigen function. Sequence comparisons permitted the characterization of few key amino acids which could be important for T cell receptor interaction and superantigen processing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Viral / genetics
  • Female
  • Hyperplasia
  • Mammary Glands, Animal / immunology
  • Mammary Glands, Animal / microbiology*
  • Mammary Glands, Animal / pathology
  • Mammary Neoplasms, Experimental / genetics
  • Mammary Neoplasms, Experimental / immunology
  • Mammary Neoplasms, Experimental / microbiology
  • Mammary Tumor Virus, Mouse / genetics
  • Mammary Tumor Virus, Mouse / immunology
  • Mammary Tumor Virus, Mouse / isolation & purification*
  • Mice
  • Mice, Inbred BALB C
  • Milk / microbiology*
  • Molecular Sequence Data
  • Neoplasm Transplantation
  • Precancerous Conditions / genetics
  • Precancerous Conditions / immunology
  • Precancerous Conditions / microbiology
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Sequence Deletion
  • Viral Proteins / genetics

Substances

  • DNA, Viral
  • Receptors, Antigen, T-Cell, alpha-beta
  • Viral Proteins

Associated data

  • GENBANK/Z22552
  • GENBANK/Z25442
  • GENBANK/Z25443
  • GENBANK/Z25444
  • GENBANK/Z25445
  • GENBANK/Z25446
  • GENBANK/Z25447
  • GENBANK/Z25448
  • GENBANK/Z25449
  • GENBANK/Z25450