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Appl Biochem Biotechnol. 1993 Jul;42(1):1-7.

Bacitracin production by a new strain of Bacillus subtilis. Extraction, purification, and characterization.

Author information

1
Mestrado de Ciencias FarmacĂȘuticas, Universidade Federal de Pernambuco, Recife-PE, Brazil.

Abstract

A new strain of Bacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of Micrococcus flavus. The production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.

PMID:
8215347
DOI:
10.1007/bf02788897
[Indexed for MEDLINE]

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