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Gene. 1994 Jun 10;143(2):301-2.

Biologically active recombinant human complement factor H: synthesis and secretion by the baculovirus system.

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Department of Biochemistry, University of Texas Health Science Center, Tyler 75710-2003.


A complete cDNA clone (4.3 kb) encoding human complement factor H (hCFH; 155 kDa) has been cloned into the pVL1393 baculovirus expression vector and transfected into Spodoptera frugiperda Sf9 insect cells. A biologically active (92 +/- 15%) 140-kDa protein was secreted into the medium with a yield of more than 5 mg/liter. This is the first report of synthesis of biologically active recombinant hCFH in a heterologous system.

[Indexed for MEDLINE]

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