Format

Send to

Choose Destination
Gene. 1994 Jun 10;143(2):301-2.

Biologically active recombinant human complement factor H: synthesis and secretion by the baculovirus system.

Author information

1
Department of Biochemistry, University of Texas Health Science Center, Tyler 75710-2003.

Abstract

A complete cDNA clone (4.3 kb) encoding human complement factor H (hCFH; 155 kDa) has been cloned into the pVL1393 baculovirus expression vector and transfected into Spodoptera frugiperda Sf9 insect cells. A biologically active (92 +/- 15%) 140-kDa protein was secreted into the medium with a yield of more than 5 mg/liter. This is the first report of synthesis of biologically active recombinant hCFH in a heterologous system.

PMID:
8206393
DOI:
10.1016/0378-1119(94)90116-3
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center