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Folia Biol (Praha). 1993;39(5):270-6.

Comparison of methods used for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines.

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1
National Drug Institute, Sofia.

Abstract

Two methods for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines were compared: the direct culture test and the DNA staining method employing bisBenzimide (Hoechst No. 33258). Contamination by different species of mycoplasma was found in 39% samples tested. It is recommended to use both techniques for a reliable detection of mycoplasma contamination.

PMID:
8206173
[Indexed for MEDLINE]
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