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Am J Physiol. 1994 May;266(5 Pt 1):G887-91.

Regulation of glucose-dependent insulinotropic peptide gene expression by a glucose meal.

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Harvard Digestive Disease Center, Division of Gastroenterology, Harvard Medical School, Boston, Massachusetts.


Glucose-dependent insulinotropic peptide (GIP) enhances insulin secretion by pancreatic beta-islet cells. Its release has been demonstrated predominantly after the ingestion of carbohydrate and fat. We have recently cloned a rat intestinal GIP cDNA and in the present studies have used this cDNA as a probe for measuring rat duodenal GIP mRNA after nutrient administration. Rats were fasted overnight, after which they were permitted to drink water or 10% glucose. The duodenum was then removed, GIP mRNA was measured by Northern hybridization analysis, and duodenal mucosa and serum GIP levels were determined by radioimmunoassay. In response to oral 10% glucose, duodenal mucosal GIP mRNA concentrations increased from 2 to 4 h, and at the end of 4 h a threefold increase was detected. Serum and mucosal GIP concentrations increased earlier, starting at 60 min, and levels remained elevated at 4 h. In separate studies, nuclear run-on assays were performed to compare the effects of glucose and water ingestion on transcription of the GIP gene. Whereas no increase in GIP gene transcription was detected in rats ingesting water, the rate of transcription doubled in glucose-fed rats. Actin gene transcription was nearly identical in both groups, indicating genomic specificity. The results of these studies indicate that duodenal GIP gene expression is stimulated at both the pre- and posttranslational levels by glucose-containing meals. These studies further suggest that the release of GIP from cellular storage granules might account for the initial increase in serum GIP levels after oral glucose ingestion.

[Indexed for MEDLINE]

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