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Gene. 1994 May 27;143(1):145-6.

Generalized and mobilizable positive-selection cloning vectors.

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Laboratory of Biotechnology, Chulabhorn Research Institute, Lak si, Bangkok, Thailand.


We have modified the positive-selection cloning vector pUN121 to expand the numbers of unique cloning sites by insertion of a multiple cloning site into the lambda cI gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzyme sites suitable for general cloning purposes. A mobilizable version (pSKM11) of pSKM10 was constructed by insertion of the IncP mob sequence which permitted mobilization of the plasmid into a wide variety of Gram- bacteria.

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