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Biochem Biophys Res Commun. 1994 May 30;201(1):346-55.

Fur (ferric uptake regulation) protein interaction with target DNA: comparison of gel retardation, footprinting and electron microscopy analyses.

Author information

1
Laboratoire d'Ecologie Microbienne, URA 147 du CNRS, Institut Gustave-Roussy, Villejuif, France.

Abstract

Fur-DNA interactions were analyzed within the regulatory regions of aerobactin and hemolysin operons by a combination of biochemical and ultrastructural methods. Cartography of the Fur binding sites, carried out from electron micrographs, agreed with the data obtained by DNase I footprinting. Visualization of the complexes confirmed the specificity and metal-dependence of Fur binding and demonstrated that the protein polymerizes on its binding sites. Such a polymerization could be involved in the repression process of the bacterial regulator.

PMID:
8198594
DOI:
10.1006/bbrc.1994.1708
[Indexed for MEDLINE]

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