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Mol Gen Genet. 1994 Apr;243(1):106-11.

High fidelity extrachromosomal recombination and gene targeting in plants.

Author information

1
Research Institute for Corp Production, Praha, Czech Republic.

Abstract

The precision of extrachromosomal homologous recombination and gene targeting in plant cells was investigated. Recombination was directed to introns of selectable marker genes where potential changes could persist without affecting the function and therefore the selectability of the genes. Approximately 9 kb of crossover regions was rescued and sequenced. Changes were detected at a frequency below one point mutation per 1000 bp, indicating that extrachromosomal recombination and gene targeting both appear to occur with high fidelity.

PMID:
8190063
DOI:
10.1007/bf00283882
[Indexed for MEDLINE]

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