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Invest Ophthalmol Vis Sci. 1994 May;35(6):2843-9.

Synthesis and transport of glutathione by cultured human retinal pigment epithelial cells.

Author information

1
Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia 30322.

Abstract

PURPOSE:

To characterize synthesis and transport of glutathione (GSH) by cultured human retinal pigment epithelial (RPE) cells.

METHODS:

Cultured human RPE cells were depleted of glutathione, then incubated with various concentrations of cysteine, glutamate, and glycine or with glutathione. High-performance liquid chromatography was used to measure intracellular glutathione with time.

RESULTS:

After depletion with diethylmaleate, intracellular glutathione was resynthesized from the amino acid precursors within 60 minutes. The addition of buthionine sulfoximine, a known inhibitor of GSH synthesis, completely eliminated the observed increase. Similar incubation with exogenous GSH failed to increase the intracellular GSH concentration.

CONCLUSION:

Cultured human RPE cells are able to rapidly synthesize glutathione from exogenously administered amino acids, but they are incapable of direct GSH uptake.

PMID:
8188479
[Indexed for MEDLINE]

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