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Mol Gen Genet. 1994 Apr;243(2):178-84.

Intragenic recombination in the CSR1 locus of Arabidopsis.

Author information

1
Department of Biology, University of Saskatchewan, Saskatoon, Canada.

Abstract

Four classes of herbicides are known to inhibit plant acetolactate synthase (ALS). In Arabidopsis, ALS is encoded by a single gene, CSR1. The dominant csr1-1 allele encodes an ALS resistant to chlorsulfuron and triazolopyrimidine sulfonamide while the dominant csr1-2 allele encodes an ALS resistant to imazapyr and pyrimidyl-oxy-benzoate. The molecular distance between the point mutations in csr1-1 and csr1-2 is 1369 bp. Here we used multiherbicide resistance as a stringent selection to measure the intragenic recombination frequency between these two point mutations. We found this frequency to be 0.008 +/- 0.0028. The recombinant multiherbicide-resistant allele, csr1-4, provides an ideal marker for plant genetic transformation.

PMID:
8177214
DOI:
10.1007/bf00280315
[Indexed for MEDLINE]

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