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Biochem Biophys Res Commun. 1994 Apr 15;200(1):435-42.

Yge1p, a eukaryotic Grp-E homolog, is localized in the mitochondrial matrix and interacts with mitochondrial Hsp70.

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Department of Chemistry, Faculty of Science, Nagoya University, Japan.


Yeast Yge1p, the gene product of YGE1, is a eukaryotic GrpE homolog found recently (Ikeda et al., 1994). We have revealed here that Yge1p is a soluble protein in the mitochondrial matrix. Depletion of Yge1p in yeast cells resulted in accumulation of the precursor of F1-ATPase beta subunit in vivo, suggesting that Yge1p is involved in protein import into mitochondria. Overexpression of Yge1p in the temperature-sensitive mutant strains of mitochondrial hsp70, Ssc1p, caused hypersensitivity to temperature for cell growth, suggesting a genetic interaction between the YGE1 and SSC1 genes. A physical interaction between Yge1p and Ssc1p was directly demonstrated by co-immunoprecipitation of Ssc1p by the anti-Yge1p antibodies. We propose that Yge1p functions in cooperation with Ssc1p in a similar manner as bacterial GrpE with DnaK.

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