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J Immunol Methods. 1994 Mar 29;170(1):117-24.

A new flow cytometric assay for the evaluation of phagocytosis and the oxidative burst in whole blood.

Author information

1
Laboratorio di Analisi, IRCCS Burlo Garofolo, Trieste, Italy.

Abstract

Phagocytes play an important role in host defence against microorganisms and different techniques are needed to evaluate their functional activities. Here we describe a rapid, simple and reliable one step procedure to measure the phagocytosis rate and oxidative burst activation of both polymorphonuclear leukocytes (PMN) and monocytes, by means of flow cytometry using only small quantities of whole blood. The two species of bacteria employed as test microorganisms, Staphylococcus aureus and Candida albicans showed a somewhat different behaviour. We found that the present method could be used as an alternative test in the diagnosis of chronic granulomatous disease (CGD). Moreover we were able to analyse, in a one step procedure, defective phagocyte functions in a group of paediatric patients suffering from recurrent microbial infections.

PMID:
8157984
DOI:
10.1016/0022-1759(94)90251-8
[Indexed for MEDLINE]

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