Format

Send to

Choose Destination
Biol Pharm Bull. 1994 Jan;17(1):136-8.

Evaluation of antiseptics by the modified phenol coefficient method: sensitivity of methicillin-resistant Staphylococcus aureus.

Author information

1
Department of Microbiology, Tokyo College of Pharmacy, Japan.

Abstract

The relationship between an effective concentration and the duration of exposure to antiseptics was evaluated in strains of Staphylococcus aureus with a known genetic background, which include methicillin-resistant strains, using a modified version of the phenol coefficient method as part of an effort to investigate the antiseptic resistance of S. aureus. Chlorhexidine digluconate killed an antiseptic-sensitive strain within 1.5 min at 22 degrees C at a standard concentration (0.1%), whereas resistant strains still survived after 1.5 min. Povidone-iodine killed the sensitive strain within 1.5 min at a concentration of 0.1%, whereas it took this agent 3.0 and 4.5 min to kill low- and high-level resistant strains, respectively, at a concentration of 0.8%. These results indicate that the modified phenol coefficient method used is suitable for the evaluation of the sensitivity of microorganisms to antiseptics. An antiseptic-resistant chain that was associated with the ebr gene exhibited cross-resistance to povidone-iodine.

PMID:
8148802
DOI:
10.1248/bpb.17.136
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center