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FEBS Lett. 1994 Mar 21;341(2-3):277-80.

Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein.

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Marine Biology Research Division, Scripps Institution of Oceanography, University of California at San Diego, La Jolla 92093.


Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.

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