Format

Send to

Choose Destination
See comment in PubMed Commons below
FEBS Lett. 1994 Mar 21;341(2-3):182-6.

cDNA cloning, expression and primary structure of Par jI, a major allergen of Parietaria judaica pollen.

Author information

  • 1Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy.

Abstract

A 659 bp cDNA clone** coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par jI since it reacted with 95% of the sera from Pj-allergic patients (n = 22) and with two Par jI-specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.

PMID:
8137937
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center