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Stain Technol. 1975 Sep;50(5):351-4.

A decalification method for ultrastructure of echinoderm tissues.

Abstract

Decalcification of echinoderm tissues for electron microscopy can be achieved after glutaraldehyde-osmium fixation by treatment with a 1:1 mixture of 2% ascorbic acid and 0.3 M NaCl for 12-24 hours. Electron photomicrographs of material decalcified by this procedure are superior to those from EDTA-treated tissue and show few of the deleteriour effects produced by EDTA.

PMID:
813335
DOI:
10.3109/10520297509117086
[Indexed for MEDLINE]

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