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Biol Chem Hoppe Seyler. 1993 Dec;374(12):1105-8.

Leucine synthesis in spinach chloroplasts: partial characterization of 2-isopropylmalate synthase.

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Botanisches Institut, Tierärztlichen Hochschule Hannover, Germany.


Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2-isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The Km values were 5 microM for acetyl-CoA and 75 microM for 2-oxoisovalerate.

[Indexed for MEDLINE]

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