DNA stretching on functionalized gold surfaces

Nucleic Acids Res. 1994 Feb 11;22(3):492-7. doi: 10.1093/nar/22.3.492.

Abstract

We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophage lambda
  • Base Sequence
  • Biotin / chemistry
  • DNA, Viral / chemistry*
  • Electromagnetic Fields
  • Gold / chemistry*
  • Microscopy, Electron
  • Molecular Sequence Data

Substances

  • DNA, Viral
  • Biotin
  • Gold