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Eur J Immunol. 1994 Mar;24(3):781-4.

In vitro development of B cells and macrophages from early mouse fetal thymocytes.

Author information

1
Howard Hughes Medical Institute, Stanford University School of Medicine.

Abstract

The developmental potentialities of early mouse fetal thymocytes were analyzed by in vitro culturing cell suspensions obtained from 12-15-gestational day thymus in contact with a bone marrow stroma clonal cell line that supports pre-B and myeloid cell differentiation. B cell and macrophage development from fetal thymocytes was observed at all fetal stages tested, but limiting dilution analysis revealed that the frequency of cells forming colonies on bone marrow stroma is the highest in the fetal thymus at day 12, then dramatically decreases until day 15. These observations suggest that the thymus rudiment is seeded by multipotential precursor cells which are not immediately committed to T cell development in the thymic cellular environment.

PMID:
8125146
DOI:
10.1002/eji.1830240345
[Indexed for MEDLINE]

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