Cloning of a complete protein-coding sequence of human platelet-type phosphofructokinase isozyme from pancreatic islet

Biochem Biophys Res Commun. 1994 Feb 15;198(3):990-8. doi: 10.1006/bbrc.1994.1141.

Abstract

We have cloned a full length protein-coding sequence of human platelet-type phosphofructokinase (PFK) from pancreatic islet cDNA library. The platelet-type PFK was composed of 784 amino acids and had a deduced molecular weight of 85,590. Homologies in the primary structure with muscle- and liver-type PFK were 71 and 67%. Clear similarities of the amino and carboxyl halves with a prokaryotic PFK indicated an evolutionary event that duplicated genes of a prototype PFK fused into larger genes of eukaryotic PFKs. Amino acid residues constituting the binding sites for various allosteric modulators were well conserved, while a couple of different residues at the inhibitory ATP sites among three isozymes may partly explain their varied degree of sensitivities to ATP. Considerable amount of platelet-type PFK expression was demonstrated in brain, heart, kidney, colon and testis.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blood Platelets / enzymology*
  • Blotting, Northern
  • Brain / enzymology
  • Cloning, Molecular
  • Colon / enzymology
  • DNA Primers
  • Female
  • Humans
  • Islets of Langerhans / enzymology*
  • Isoenzymes / biosynthesis
  • Isoenzymes / blood
  • Isoenzymes / genetics*
  • Kidney / enzymology
  • Male
  • Molecular Sequence Data
  • Myocardium / enzymology
  • Organ Specificity
  • Phosphofructokinase-1 / biosynthesis
  • Phosphofructokinase-1 / blood
  • Phosphofructokinase-1 / genetics*
  • Placenta / enzymology
  • Polymerase Chain Reaction
  • Pregnancy
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Rats
  • Sequence Homology, Amino Acid
  • Testis / enzymology

Substances

  • DNA Primers
  • Isoenzymes
  • RNA, Messenger
  • Phosphofructokinase-1

Associated data

  • GENBANK/D25328