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J Virol Methods. 1993 Dec 15;45(2):229-33.

A liquid, colorimetric presence-absence coliphage detection method.

Author information

1
Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061-0404.

Erratum in

  • J Virol Methods 1994 Jul;48(2-3):349.

Abstract

A liquid, colorimetric presence-absence coliphage detection method based on the induction of beta-galactosidase by Escherichia coli is described. The release of beta-galactosidase in the medium due to lytic cell infections by coliphages permits the hydrolysis of a yellow chromogenic substrate that develops into a distinct red coliphage positive sample, while a coliphage negative sample remains yellow. This method has proven to be rapid, simpler to perform than an agar medium assay, easy to read and interpret, inexpensive, and highly sensitive.

PMID:
8113347
DOI:
10.1016/0166-0934(93)90106-2
[Indexed for MEDLINE]

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