Format

Send to

Choose Destination
Hum Genet. 1994 Feb;93(2):125-8.

Assignment of human satellite 1 DNA as revealed by fluorescent in situ hybridization with oligonucleotides.

Author information

1
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.

Abstract

We have used a fluorescent in situ hybridization procedure to detect human satellite 1 DNA, the simple sequence family that constitutes the non-male-specific fraction of classical satellite 1 DNA. Satellite 1 appears to be located on pericentromeric regions of chromosomes 3, 4 and 13, and on satellites of each acrocentric chromosome. These results suggest a possible relationship between quinacrine fluorescence of heterochromatin and DNA composition. Furthermore, by means of multicolour in situ hybridization, we have spatially resolved satellite 1 sequences and centromeric alpha-satellite within heterochromatic blocks.

PMID:
8112734
DOI:
10.1007/bf00210595
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center