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Nat Genet. 1993 Sep;5(1):17-21.

High resolution visual mapping of stretched DNA by fluorescent hybridization.

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Cancer Therapy and Research Center, San Antonio, Texas 78229.


We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi-colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a > 200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.

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