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Appl Microbiol. 1975 Sep;30(3):445-9.

Liquid nitrogen cryo-impacting: a new concept for cell disruption.

Abstract

High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells.

PMID:
810088
PMCID:
PMC187201
[Indexed for MEDLINE]
Free PMC Article

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