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J Immunol Methods. 1994 Sep 14;174(1-2):231-5.

Determination of arginase activity in macrophages: a micromethod.

Author information

1
Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.

Abstract

We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25,000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine;

PMID:
8083527
[Indexed for MEDLINE]

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