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FEMS Microbiol Lett. 1994 Aug 1;121(1):93-8.

Individualisation of two new genomic groups among American Borrelia burgdorferi sensu lato strains.

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Laboratoire de Bactériologie, Faculté de Médecine Cochin-Port Royal, Paris, France.


We developed a quick typing method for Borrelia burgdorferi sensu lato strains using a fla gene-based PCR assay, followed by dot blot hybridization with non-radioactive species-specific probes. Thirty-six out of 46 strains belonged to one of the four described species (B. burgdorferi sensu stricto n = 11, B. garinii n = 11, B. afzelii n = 9 and B. japonica n = 5) and hybridized with its own species-specific probe. Among the 10 remaining American strains, two new additional genomic groups were identified. This finding was confirmed by direct sequencing of the fla gene-derived amplicons and whole DNA hybridization.

[Indexed for MEDLINE]

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