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Microsc Res Tech. 1994 Jun 15;28(3):165-77.

Scanning and transmission electron microscopic studies of normal and diabetic acellular glomerular and retinal microvessel basement membranes.

Author information

1
Department of Anatomy and Cell Biology, University of North Dakota, Grand Forks 58202.

Abstract

Basement membranes (BMs) were first described in the mid-19th century, but they were not isolated and prepared for compositional studies until nearly 100 years later. Early methods of isolation were carried out on renal glomeruli, which were first sub-fractionated from kidney tissues by sieving. BMs were then isolated from the glomeruli by ultrasonic disruption, which, following low speed centrifugation, yielded "purified" but highly fragmented BM material. In an effort to obviate the mechanical damage to BMs produced by ultrasound, a sequential detergent solubilization technique was introduced that resulted in morphologically intact BMs from a variety of tissue sub-fractions. This was highly advantageous because "acellular" BMs produced by the procedure could be examined critically by light and electron microscopic methods. Subsequently, this procedure has been utilized to demonstrate the substructural heterogeneity of vascular and non-vascular BMs from a wide variety of animal species. The current review describes the results of scanning and transmission electron microscopic studies of acellular BMs prepared from renal glomeruli and from the retinal microvessels of the eye. These BMs are of particular interest to basic scientists and clinicians because they are altered in several disease states, most notably diabetes mellitus. An effort is made to point out the implications of glomerular and retinal vessel BM changes to the pathogenesis of diabetic kidney and retinal vessel BM disease.

PMID:
8068980
DOI:
10.1002/jemt.1070280302
[Indexed for MEDLINE]

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