Format

Send to

Choose Destination
Proteins. 1994 May;19(1):77-9.

Expression, purification, and crystallization of restriction endonuclease PvuII with DNA containing its recognition site.

Author information

1
W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, New York 11724.

Abstract

We have overexpressed the type II restriction endonuclease PvuII (R-PvuII) in E. coli, prepared large amounts of the homogeneous enzyme, and crystallized it with an oligonucleotide carrying a PvuII recognition site. The cocrystals are orthorhombic space group P2(1)2(1)2(1) with cell constants a = 95.8 A, b = 86.3 A, c = 48.5 A, and diffract X-rays to at least 2.7 A. There is a complex of two protein subunits and one oligonucleotide duplex in the asymmetric unit.

PMID:
8066089
DOI:
10.1002/prot.340190110
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center