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J Mol Biol. 1994 Aug 19;241(3):480-2.

Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli.

Author information

1
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.

Abstract

Human platelet profilin was expressed in Escherichia coli using a T7 based expression vector. The recombinant material is similar to authentic human platelet profilin based on the measured Kd for rabbit skeletal muscle actin. Crystals of the recombinant material were obtained from both PEG 8000 and (NH4)2SO4. These crystals are isomorphous and belong to the monoclinic space group C2, a = 75.0, b = 32.0, c = 62.5, beta = 123 degrees. These crystals contain one molecule in the asymmetric unit and diffract to at least 2.0 A.

PMID:
8064860
DOI:
10.1006/jmbi.1994.1522
[Indexed for MEDLINE]

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